Fig. 1

Heat map of miRNA and lncRNA differential expression during the CN-induced osteoblast differentiation of MSCs. a In total, 77 miRNAs were differentially expressed, including 36 that were upregulated and 41 that were downregulated. b In total, 295 lncRNAs were differentially expressed, including 281 that were upregulated and 14 that were downregulated. c qRT-PCR verification of the miRNA microarray results. Compared with the control group, the expression levels of miR-365, miR-330 and miR-124 were higher in CN-induced MSCs, and those of miR-483, miR-298, miR-361 and miR-296 were lower in CN-induced MSCs, which was consistent with the miRNA microarray results. d qRT-PCR verification of the lncRNA microarray results. Compared with the control group, the expression levels of NR_046246.1, NR_046239.1, XR_086062.1, and XR_145872.1 were higher in CN-induced MSCs, and those of NR_031896.1, XR_146480.1 and XR_145894.1 were lower in CN-induced MSCs, which was consistent with the lncRNA microarray results. P < 0.05, compared to the control group. The color scale shown on the top illustrates the relative expression level of the genes between two group: red denotes high expression levels, whereas green denotes low expression levels