Fig. 7

Vdr overexpression rescues the inhibitory effect of miR-298 during the CN-induced osteoblast differentiation of MSCs. a AR-S showed that more calcium mineral deposition was found in the CN + pcDNA 3.1-Vdr + miR-298 mimic group than in the CN group, while less calcium mineral deposition was found in the CN + pcDNA 3.1-Vdr group. Thus, pcDNA 3.1-Vdr rescued the inhibitory effect of miR-298 during the CN-induced osteoblast differentiation of MSCs. b & c Western blot detection of the protein expression of osteogenic-related factors in MSCs transfected with miR-298 mimic or pcDNA 3.1-Vdr; the results were similar to the AR-S results. Control group: MSCs transfected without pcDNA 3.1-Vdr or miR-298 mimic; pcDNA 3.1-Vdr group: MSCs transfected with pcDNA 3.1-Vdr; pcDNA 3.1-Vdr + miR-298 mimic group: MSCs co-transfected with pcDNA 3.1-Vdr and miR-298 mimic. *P < 0.05 vs. the CN group. #P < 0.05 vs. the miR-298 mimic group