Fig. 1

NE stimulation triggers an acute and broad transcriptional response in human adipocytes. a Workflow diagram for sample preparation. Adipocyte precursor cells were isolated from donors (n = 4), differentiated and treated with NE or vehicle for 3 h, harvested and subsequently analyzed by RNA-seq (The female human silhouette was modified from a clipart with a public domain license creative commons CC0). b Principal component analysis (PCA) and unsupervised hierarchical clustering (c) showed clustering between stimulated (orange) and unstimulated (blue) adipocytes in two distinct groups, suggesting that NE stimulation causes deep changes in the transcriptome. Columns are biological replicates and rows represent differentially expressed genes. d Reactome pathway enrichment for up- and e down-regulated DEGs after acute NE stimulation