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Fig. 2 | BMC Genomics

Fig. 2

From: High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division

Fig. 2

High-throughput DNA sequencing of plasmid libraries from the reference-unsorted and filamentous-sorted populations. a Comparison of the distribution of mapped reads in 10 kb UTI89 chromosomal bins, showing the degree of enrichment of mapped reads in specific bins after flow cytometry. The dotted line indicates 20% of the mapped reads (see text). b The distribution of read depth along the ~ 5.06 Mbp UTI89 chromosome in the filamentous-sorted sample (red) and the reference-unsorted sample (blue), with a bin size of 1 kb. c The proportion of enriched regions found in common in both biological replicates versus their corresponding threshold significance score (−log10[P-value]) (left-hand y-axis), and the total number of enriched regions from both replicates (right-hand y-axis). The score of 70 (dotted line) represents the selected threshold criteria for display in Table 2. d The number of reads mapped to the region 1,295,150 – 1,302,200 bp of UTI89 in the filamentous-sort (red) and the reference-unsort (blue) samples within a bin window of 50 bp. Large read depth numbers in the filamentous-sort sample (replicate 1, red) can be seen against the reference-unsorted sample in the region spanning from the ycgI homolog to the minCDE genes (the region identified by MACS is shown by the bracket). Open reading frames (grey boxes) encoded by the upper and lower strands are indicated by their relative positioning below the axis

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