Fig. 1
T. cruzi TcII field isolates: geographic distribution and phylogenomic analysis based on nuclear markers. a From the seven TcII samples evaluated, five were isolated from the northeast region of Minas Gerais: S11 (Itaipé - 17.3339o S, 41.6411o W), S154a (Itaipé - 17.3339o S, 41.6411o W), S23b (Porteirinha - 15.7032o S, 43.0583o W), S44a (Turmalina − 17.2856o S, 42.7269o), and S92a (Teófilo Otoni - 17.8600o S, 41.5091o W); while two were obtained from the central region of Minas Gerais: S15 (Felixlândia - 18.7136o S, 44.9253o W) and S162a (Congonhas do Norte - 18.8242o S, 43.6759o W). b Maximum likelihood phylogenomic analysis of the TcII field isolates and previously sequenced T. cruzi from different DTUs, based on 794 nuclear single copy conserved genes. The colored circles correspond to the geographic localization in which each TcII isolate was obtained. The numbers correspond to the percentage of bootstrap replicates that supported each branch, where 100% corresponds to the 1,000 replicates c PCA plot based on the SNPs present in the seven TcII field isolates. Ync = Y strain non-cloned population; CLB-Esmo = CL Brener Esmeraldo-like haplotype; CLB-Nonesmo = CL Brener Non-esmeraldo-like haplotype