Fig. 4

Predicted ploidy of T. cruzi field isolates. a The predicted ploidy of each chromosome from the T. cruzi field isolates S11, S154a, S162a, S15, S92, S23b, S44a, using as a reference the 41 CL Brener chromosome sequences, was estimated based on the median coverage of all T. cruzi genes, excluding those belonging to the largest multigene families. Each bar corresponds to the ratio between the genes median RDC in the chromosome and the genome coverage, representing its predicted chromosome copy number. Dark-blue, light blue, black, yellow, orange, red and brown bars correspond, respectively, to chromosomes with statistically supported predicted somies of 1; 1.5; 2; 2.5; 3; 3.5; 4. b The overall genomic ploidy of each strain was estimated by allele frequency of heterozygous SNPs, where a tendency of 0.4–0.5 represents a predominantly diploid genome. c Comparison of the predicted ploidy of each chromosome among TcII strains. Each line corresponds to a TcII strain