Fig. 1

Transposon insertion and its effect on gene expression. (a) Insertion of a PiggyBac Transposon in the 5′ upstream region the gene k13 (b) results in the gene being aberrantly down-regulated at the 6 h time point and up-regulated at the 24 h time point. The expression of k13’s same strand neighbors are unaffected by the insertion. The changes in K13 expression are consistent with the known regulation of the calmodulin promoter in P. falciparum. The transcript expression is measured in fragments per kilobase per million mapped reads (FPKM). The abbreviations for the piggybac transposon are: inverted terminal repeat 1 (ITR1), histidine-rich protein-2 (hrp2), human dihydrofolate reductase (hdhfr), regulatory elements of calmodulin (cam), and inverted terminal repeat 2 (ITR2). The insertion occurs 1034 nucleotides up-stream of k13 (see Additional file 1: S1 for a finer resolution mapping of the insertion site)