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Fig. 1 | BMC Genomics

Fig. 1

From: RRAD, IL4I1, CDKN1A, and SERPINE1 genes are potentially co-regulated by NF-κB and p53 transcription factors in cells exposed to high doses of ionizing radiation

Fig. 1

Activation of NF-κB and p53 pathways in U-2 OS cells exposed to a single high dose of ionizing radiation. a Kinetics of IR-induced nuclear translocation of RelA and p53 analyzed by Western blot in nuclear extracts (nuclear protein NPM3 was used as a loading reference). b Kinetics of RelA and p53 binding in the promoter of CXCL8 and CDKN1A gene, respectively, analyzed by ChIP-qPCR. c Kinetics of IR-induced activation of CXCL8 and CDKN1A gene expression analyzed by RT-qPCR. d Total levels of RelA and p53 proteins in cells transfected with specific siRNA, either untreated or 4 h. after 10 Gy irradiation, analyzed by Western blot in whole cell lysates

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