Fig. 4

K-mer and read mapping overview. a KAT density plots demonstrating the 21-mer multiplicity of read 1 (x axis) against read 2 (y axis) for P. effusa R13, P. effusa R14, P. tabacina J2 and P. tabacina S26. Approximate locations of homozygous and heterozygous 21-mer are labeled on the x-axis. b KAT spectra-cn plots of the 21-mer multiplicity of the read set (x axis) against their respective assembly (y axis) for P. effusa R13, P. effusa R14, P. tabacina J2 and P. tabacina S26. Black areas under the peaks represent 21-mers present in the reads, absent in the assembly, red indicates 21-mers are present once in the assembly, purple 21-mers are present twice, green thrice. c Frequency of reads supporting the alternative allele over the entire assembly and gene space for P. effusa R13 and P. effusa R14. The allele frequency is cut-off at 0.2 and 0.8 on the x-axis of all plots. d Normalized read depth of every gene predicted in both P. effusa R13 and P. effusa R14. The height of each plot indicates the number of gene models at the normalized coverage displayed on the x axis