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Fig. 7 | BMC Genomics

Fig. 7

From: Dissecting clinical outcome of porcine circovirus type 2 with in vivo derived transcriptomic signatures of host tissue responses

Fig. 7

Superinfection increases PCV2 replication through a macrophage-lymphoblast paracrine signaling axis. a ImmuneSigDB gene set expression in the PMWS field study (FDR < 0.01 and opposite expression of each pairwise phenotype). The average expression of the leading-edge genes in every gene set (genes that contribute to the enrichment) are displayed for each patient sample. Bars next to each gene set indicate the signed FDR for its enrichment in log10 scale. PMWS versus healthy lymph node comparison displays a dramatic repression of lymphocyte gene expression signatures, and induction of myeloid cell signatures. b Experimental set-up of PPL-macrophage co-culture system mimicking PMWS lymph nodes. c PCV2-inoculated PPLs were seeded on a porous insert with macrophages at the bottom of the well. Macrophages were additionally challenged with PRRSV at 0 h. d Relative PPL infection levels at 72 hpi. Means ± sd represent two independent experiments in triplicate (n = 6; *P < 0.05, two-tailed Mann-Whitney)

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