Table 1 Sequencing, pre-processing of reads and assembly of the Ulva compressa transcriptome
Libraries | Sequence reads | Reads after QC | Reads after decontamination |
|---|---|---|---|
Sequencing, quality control and decontamination of reads | |||
0 h-1 | 11,438,569 | 11,152,558 (97.50%) | 10,861,476 (94.95%) |
3 h-1 | 20,650,530 | 15,993,724 (77.45%) | 15,768,212 (76.36%) |
3 h-2 | 20,884,112 | 18,978,764 (90.88%) | 18,756,712 (89.81%) |
6 h-1 | 21,666,034 | 19,744,705 (91.13%) | 19,436,688 (89.71%) |
6 h-2 | 19,419,660 | 17,691,271 (91.10%) | 17,383,443 (89.51%) |
12 h-1 | 17,354,946 | 15,909,292 (91.67%) | 15,785,199 (90.96%) |
12 h-2 | 16,308,426 | 12,485,489 (76.56%) | 12,360,634 (72.79%) |
24 h-1 | 12,467,371 | 12,150,061 (97.45%) | 11,862,105 (95.15%) |
Transcriptome assembly | |||
Total genes | 64,191 | ||
Total transcripts | 106,704 (92.57 Mb) | ||
Total bases | 92,575,573 (92.57 Mb) | ||
Min sequence lenght | 200 | ||
Max sequence lenght | 9118 | ||
Average sequence lenght | 868 | ||
Median sequence lenght | 542 | ||
N25 lenght | 2,28 | ||
N50 lenght | 1383 | ||
N75 lenght | 716 | ||
N90 lenght | 349 | ||
N95 lenght | 261 | ||
A | 20.95% | ||
T | 20.87% | ||
G | 29.18% | ||
C | 29.00% | ||
(A + T) | 41.82% | ||
(G + C) | 58.18% | ||
N | 0.00% | ||
Transcriptome completeness | |||
Completeness | 89.59% | ||
Complete CEG | 265 | ||
Complete and single copy | 123 | ||
Complete and multi-copy | 142 | ||
Fragmented CEGs | 85 | ||
Missing CEGs | 79 | ||
Total CEGs database | 429 | ||