A transcriptomic snapshot of early molecular communication between Pasteuria penetrans and Meloidogyne incognita

Table 7 Comparison of fold expression of selected transcripts between RNA-Seq and qRT PCR experiments. The P values for the RNA-Seq data is provided in parentheses, and the statistical significance of the qRT PCR data is indicated by superscripted letters (a,b)

S. No.	Transcript ID	Annotation	Fold Change
S. No.	Transcript ID	Annotation	RNA-Seq	qRT PCR
1.	TR11426	Heat shock protein 20	−4.18 (P = 0.02)	−1.24
2.	TR14120	Phospholipase A2	−2.78 (P = 0.01)	−7.98^b
3.	TR16177	Aspartic protease	−3.93 (P = 0.00)	−2.52^b
4.	TR10194	Bm 3887	−2.10 (P = 0.05)	−3.20^b
5.	TR10010	Fructose bis phosphate aldolase	−3.60 (P = 0.00)	−7.63^b
6.	TR31579	Selenium binding protein	2.01 (P = 0.00)	6.89^b
7.	TR14793	Glucosyl transferase	2.34 (P = 0.04)	4.89^b
8.	TR23171	Venom allergen-like protein	2.68 (P = 0.02)	1.73^a
9.	TR35213	Glycoside hydrolase	−3.89 (P = 0.00)	−4.16^b
10.	TR40461	Glutathione S-transferase	2.13 (P = 0.00)	0.51
11.	TR11544	Fatty acid and retinol binding protein	−5.06 (P = 0.00)	−6.09^b
12.	TR26363	Major sperm protein	3.46 (P = 0.00)	2.92^b
13.	TR10990	Ubiquitin	− 4.82 (P = 0.00)	−5.04^b
14.	TR20164	Tropomyosin	−3.32 (P = 0.00)	−0.35
15.	TR24005	Serine protease	−3.96 (P = 0.00)	−2.71^b

^asignificant and ^bhighly significant as compared to control. Data was considered statistically significant at P value ≤0.05 for both the experiments

ISSN: 1471-2164