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Fig. 3 | BMC Genomics

Fig. 3

From: Important cardiac transcription factor genes are accompanied by bidirectional long non-coding RNAs

Fig. 3

Different expression patterns of Tbx5ua and Tbx5. a RefSeq genome annotation of Tbx5 locus. The isoforms of Tbx5 and Tbx5ua that were identified in our RNA-seq analysis were labeled with isoform numbers. The qPCR primers used to quantify Tbx5 and Tbx5ua are indicated as arrowheads. b The log10-ratios of intact/nuclear RNA abundances. Gapdh and Neat1 serve as controls for cytoplasmic and nuclear localized RNA, respectively. c The expression levels of Tbx5 and Tbx5ua during development as determined by qRT-PCR. In the ventricle, the expression level of Tbx5ua is increased with the progression of development while that of Tbx5 is decreased (n = 3). d The expression levels of Tbx5 and Tbx5ua in the left and right ventricles at E11.5 as determined by qRT-PCR. Unlike Tbx5, Tbx5ua was equally expressed in both ventricles (n = 3, *: p < 0.05, Welch's t-test). e Schematic diagram of the Tbx5ua knockdown experiment. Three tandem copies of bovine growth hormone polyadenylation signal were inserted along with the neomycin resistance gene (NeoR) or EGFP. The selection markers were subsequently removed with cell-permeable Cre recombinase

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