Table 1 Primer sequences used for RT-qPCR amplifications of reference gene and selected genes in porcine expanded blastocysts
Genes | Primer sequences (5′ → 3′) | GenBank Accession no. | Product size (bp) | Amplification efficiency (%) |
|---|---|---|---|---|
CDK7 | (F) TGGAATCCCGCTACAACATATC | XM_021076538.1 | 111 | 0.997 |
(R) AATGCCTGTGTGGCTGTAA | ||||
SPCS1 | (F) TGGATTACAAGGGCCAGAAG | NM_001114288.2 | 91 | 0.996 |
(R) GCCACGTACCCGTAGATAAAT | ||||
GADD45GIP1 | (F) ATTGAAGAGTGCATGGCTAAGA | XM_003123339.3 | 89 | 0.999 |
(R) TTGTCTGCTTGCTCCTTCTC | ||||
ANAPC1 | (F) CCTGTTTCCTTGTCTACCACTC | XM_013995810.1 | 118 | 0.999 |
(R) ACTGGCATCTTGAGCTGTTTA | ||||
HDAC1 | (F) GGGATTGATGACGAGTCCTATG | XM_013999116.2 | 104 | 0.999 |
(R) GAGTCAGAGCCACACTGTAAG | ||||
CUL1 | (F) CAGTTACTCGGAGAAGTCCTAAC | XM_013979868.1 | 117 | 0.999 |
(R) ACCATCAACTCGCTCCAAATA | ||||
SRP9 | (F) CCTGCCCAATTCTCCCTTTAT | XM_003130540.6 | 90 | 0.994 |
(R) CAATCCCATACTTCCGGTTTACT | ||||
HAT1 | (F) GCAGTAGAGGCTCAACAGAAG | XM_003483674.4 | 91 | 0.999 |
(R) CACTCATGTCAGTTACCAGTAGTC |