Fig. 5

Profile alignments of the nicotine content and MRP2A and MRP2B transcripts in N. rustica (Nrus) (a) and N. tabacum TN90 (b) in the roots (Root YP), and lower (LL YP) and upper (UL YP) leaves of young plants grown in the greenhouse, in the roots (Root Field), lower (LL Field) and upper (UL Field) leaves of plants grown in the field, and finally in the roots (Root FP), flower (Flower FP) and lower (LL FP) and upper (UL FP) leaves of flowering plants grown in the greenhouse. Profiles of asparagine (Asn), glutamine (Gln), aspartate (Asp) and glutamate (Glu) contents (mg/g DW) in N. rustica (Nrus) and its progenitors, N. paniculata (Npan), N. knightiana (Nkni) and N. undulata (Nund) (c) and N. tabacum (Ntab) and its progenitors, N. tomentosiformis (Ntom) and N. sylvestris (Nsyl) (d). Data were collected from flowering plants in the greenhouse. Sodium (Na) and potassium (K) profiles in N. rustica and its progenitors (e and f) as well as in N. tabacum and its progenitors (g and h). Data were collected in young plants grown in the greenhouse, but root data are missing for N. undulata. LL: lower leaf, UL: upper leaf, R: root, FL: flower, GH_1: greenhouse pre-flowering, FI_1: field pre-flowering and GH_2: greenhouse flowering. In graphic a, b, f and h, curves were aligned to ease comparison independently of the calculated correlations