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Table 2 Akaloid concentration from plants grown in the field, young plants grown in the greenhouse, and flowering plants grown in the greenhouse

From: The impact of genome evolution on the allotetraploid Nicotiana rustica – an intriguing story of enhanced alkaloid production

  Nicotine [mg/g] Nornicotine [μg/g] Anatabine [μg/g] Nitrate [mg/g] Asn [mg/g] Gln [mg/g] Asp [mg/g] Glu [mg/g]
mean stdev mean stdev mean stdev mean stdev mean stdev mean stdev mean stdev mean stdev
Field
N. rustica Lower leaf 47.7 9.2 968.4 357.1 967.4 116.0 7.9 11.2 0.2 0.1 0.8 0.4 0.4 0.1 0.9 0.3
Upper leaf 34.4 15.5 576.1 276.9 529.0 263.8 3.5 2.7 0.3 0.2 2.0 1.5 0.5 0.1 1.1 0.3
Root 14.5 1.4 558.6 47.0 950.7 101.6 6.5 1.0 0.4 0.1 1.2 0.4 0.4 0.1 1.1 0.2
N. paniculata Lower leaf 5.0 0.6 495.2 56.1 39.0 3.9 8.9 3.4 0.2 0.1 1.4 0.3 0.2 0.0 0.5 0.1
Upper leaf 4.2 1.0 515.5 162.9 29.4 6.2 4.9 2.6 0.4 0.1 4.8 1.6 0.5 0.2 0.6 0.1
Root 7.9 1.7 4424.5 1238.4 271.2 81.7 4.5 0.9 0.4 0.1 1.5 0.3 0.1 0.0 1.0 0.2
N. knightiana Lower leaf 7.8 1.8 573.2 175.2 61.1 9.2 4.4 3.8 0.1 0.1 0.7 0.4 0.1 0.1 0.4 0.1
Upper leaf 2.7 0.4 203.6 17.2 18.1 3.2 1.6 1.0 0.2 0.1 1.6 0.6 0.4 0.1 1.0 0.2
Root 4.6 1.3 5061.3 1487.9 336.4 61.1 5.3 1.7 0.7 0.3 2.1 0.3 0.3 0.2 1.4 0.2
N. undulata Lower leaf 7.3 1.2 464.6 151.1 49.3 10.8 23.5 11.9 0.1 0.1 0.5 0.3 0.2 0.1 0.7 0.3
Upper leaf 5.9 1.0 475.5 107.5 30.3 6.3 9.7 9.6 0.3 0.1 1.9 0.5 0.4 0.2 0.7 0.3
Root 16.8 2.2 2614.6 435.3 383.9 22.9 11.9 4.0 0.4 0.1 3.0 0.6 0.6 0.2 1.6 0.4
N. tabacum Lower leaf 25.6 5.7 787.2 217.5 1041.8 271.0 1.3 1.0 0.1 0.0 0.6 0.4 0.1 0.1 0.6 0.1
Upper leaf 5.0 0.4 138.0 29.5 168.5 29.5 2.7 1.7 0.5 0.3 4.4 2.3 0.2 0.1 1.2 0.5
Root 11.1 1.5 823.1 130.2 1154.1 168.4 4.6 3.2 0.2 0.1 1.4 0.3 0.1 0.0 0.7 0.2
N. sylvestris Lower leaf 10.7 1.9 1566.1 970.3 198.6 25.3 7.7 7.6 1.3 1.6 2.3 2.2 0.3 0.2 0.4 0.1
Upper leaf 3.9 1.2 643.2 496.0 91.9 49.5 3.7 3.2 3.3 3.0 4.8 3.9 0.4 0.3 0.7 0.2
Root 13.8 1.7 878.7 130.0 1355.1 320.8 8.8 2.5 0.8 0.4 2.1 0.7 0.2 0.1 0.8 0.3
N. tomentosiformis Lower leaf 0.0 0.0 783.9 96.8 131.1 24.3 0.8 1.0 0.2 0.1 0.8 0.2 0.1 0.0 0.5 0.2
Upper leaf 0.0 0.0 435.2 66.1 65.1 17.1 2.0 2.2 0.3 0.1 0.8 0.4 0.2 0.1 0.8 0.2
Root 3.5 0.3 1173.9 161.7 1703.1 62.0 6.8 3.6 0.5 0.2 1.8 0.3 0.3 0.1 1.3 0.2
Greenhouse -- young plants
N. rustica Lower leaf 15.1 2.1 201.8 46.9 235.5 117.0 31.1 1.7 0.3 0.1 0.8 0.1 0.8 0.2 1.6 0.2
Upper leaf 27.0 5.0 304.9 41.4 173.4 44.1 13.6 4.3 0.6 0.1 2.2 0.6 1.2 0.1 2.1 0.2
Root 7.2 1.0 240.4 41.0 421.3 122.0 11.3 3.0 0.3 0.0 0.5 0.0 2.0 0.2 0.8 0.1
N. paniculata Lower leaf 4.5 0.4 702.4 220.7 92.2 122.6 52.4 19.2 0.3 0.2 2.0 0.6 0.6 0.2 1.4 0.3
Upper leaf 4.7 1.1 629.4 152.5 11.3 9.3 23.4 9.5 0.2 0.1 3.4 1.3 1.1 0.4 2.2 0.4
Root 5.5 0.9 3543.3 1117.7 67.5 43.1 10.3 2.7 0.2 0.1 0.2 0.0 1.2 0.3 0.8 0.1
N. knightiana Lower leaf 8.2 2.3 430.4 155.6 66.4 20.6 9.3 6.4 0.1 0.0 1.1 0.2 0.5 0.1 1.1 0.2
Upper leaf 7.6 3.1 536.8 244.9 19.3 9.4 3.6 2.8 0.1 0.0 1.3 0.3 0.7 0.1 2.0 0.2
Root 6.7 0.9 2861.5 783.4 155.9 15.5 5.1 1.4 0.6 0.2 0.2 0.0 2.7 0.2 0.9 0.1
N. undulata Lower leaf 5.0 2.1 84.3 37.4 52.6 27.2 74.0 33.3 0.1 0.0 1.8 1.2 0.3 0.1 1.0 0.3
Upper leaf 10.6 6.1 187.4 96.2 78.1 32.3 20.7 7.6 2.2 3.0 3.8 0.9 0.9 0.1 2.6 0.8
Root 5.6 2.2 682.1 230.8 371.6 180.9 12.2 2.0 0.1 0.0 0.4 0.1 1.9 1.1 1.0 0.3
N. tabacum Lower leaf 10.8 2.1 289.0 64.9 350.8 44.3 1.0 0.7 0.1 0.0 0.6 0.1 0.3 0.0 1.5 0.2
Upper leaf 6.1 1.2 166.6 29.9 129.4 31.9 2.5 0.6 0.2 0.0 3.5 0.4 0.9 0.1 2.0 0.2
Root 4.0 0.4 244.7 34.4 292.2 21.2 2.8 0.4 0.1 0.0 0.2 0.0 1.4 0.3 0.5 0.1
N. sylvestris Lower leaf 6.3 1.3 225.6 154.5 35.6 9.3 7.8 5.9 0.1 0.0 0.8 0.6 0.2 0.1 0.6 0.2
Upper leaf 4.7 0.8 128.5 40.1 34.2 7.4 6.1 2.1 0.1 0.0 2.5 0.5 0.7 0.2 1.3 0.2
Root 8.1 1.4 777.7 218.9 360.5 32.0 4.0 1.3 0.1 0.0 0.3 0.1 1.5 0.5 0.8 0.1
N. tomentosiformis Lower leaf 0.0 0.0 770.2 176.8 140.4 30.3 1.2 1.9 0.2 0.0 1.3 0.4 0.6 0.1 1.3 0.4
Upper leaf 0.0 0.0 450.1 159.0 77.0 25.5 2.5 1.6 0.4 0.1 5.3 0.9 1.4 0.2 1.6 0.2
Root 1.7 0.5 352.2 101.6 639.2 129.3 6.2 1.7 0.7 0.2 0.5 0.1 2.4 0.4 1.0 0.2
Greenhouse -- flowering plants
N. rustica Lower leaf 24.7 8.6 400.6 418.9 571.5 338.2 34.7 6.0 0.6 1.0 1.2 0.5 0.6 0.4 1.9 0.8
Upper leaf 30.5 9.5 448.5 72.8 642.6 417.4 32.7 7.2 0.4 0.5 1.5 0.2 0.7 0.3 1.4 0.4
Root 7.8 2.5 313.9 99.4 514.4 109.1 9.9 4.7 0.5 0.3 0.5 0.1 1.4 0.6 0.8 0.2
Flower 5.8 4.6 193.8 41.9 68.3 44.9 1.1 0.5 1.6 1.3 1.3 0.3 0.4 0.0 0.7 0.1
N. paniculata Lower leaf 6.4 3.7 747.6 449.5 134.4 204.9 50.7 18.6 0.1 0.0 1.1 0.5 0.2 0.1 0.7 0.4
Upper leaf 2.0 0.8 221.7 88.9 0.0 0.0 38.0 8.9 0.3 0.2 3.2 2.1 1.5 0.7 2.3 0.3
Root 5.4 2.2 3620.3 1363.9 67.0 35.5 4.9 1.3 0.2 0.1 0.1 0.0 1.0 0.3 0.5 0.1
Flower 1.8 0.8 162.5 78.3 0.0 0.0 2.0 0.8 2.5 0.7 6.6 2.8 0.5 0.1 1.4 0.2
    N. knightiana Lower leaf 6.8 0.9 264.7 76.4 122.7 15.3 10.1 3.8 0.0 0.0 0.3 0.0 0.2 0.1 1.3 0.5
Upper leaf 4.4 1.1 314.3 95.7 30.9 38.9 13.3 4.1 0.2 0.1 1.0 0.1 1.4 0.5 2.0 0.5
Root 7.5 1.8 5199.8 1522.4 289.1 117.1 5.8 1.4 1.0 0.5 0.2 0.2 2.2 0.7 0.7 0.3
Flower 0.9 0.4 26.7 10.3 0.0 0.0 0.8 0.3 1.7 0.4 5.0 1.8 0.5 0.2 1.2 0.3
N. undulata Lower leaf 16.3 5.7 650.0 207.5 258.9 174.3 18.9 12.2 0.1 0.1 0.6 0.2 0.5 0.1 1.0 0.1
Upper leaf 9.4 0.3 702.9 360.1 77.3 48.7 6.5 1.7 0.0 0.0 0.4 0.0 0.6 0.1 0.9 0.1
Root 7.6 2.3 2112.3 1042.3 379.9 212.2 17.4 2.4 0.6 0.0 0.8 0.3 3.0 0.6 1.0 0.4
Flower 0.3 0.1 70.3 5.4 0.0 0.0 0.3 0.1 1.9 0.5 8.3 2.4 1.6 0.2 1.1 0.4
N. tabacum Lower leaf 10.0 2.6 323.5 67.6 429.9 90.2 1.7 0.9 0.1 0.2 0.4 0.1 0.4 0.1 1.1 0.2
Upper leaf 8.2 1.9 251.5 79.4 290.1 77.7 3.9 2.0 0.1 0.0 0.8 0.1 0.9 0.1 2.2 0.6
Root 4.8 0.4 425.1 53.6 380.9 34.0 1.6 0.4 0.3 0.1 0.1 0.0 1.3 0.3 0.3 0.1
Flower 2.1 0.3 65.6 29.1 36.5 8.3 7.5 1.2 10.7 1.8 14.5 3.2 1.2 0.1 1.4 0.2
N. sylvestris Lower leaf 4.1 0.3 178.3 38.1 31.0 10.1 12.6 6.3 0.1 0.1 0.3 0.1 0.2 0.1 0.5 0.3
Upper leaf 2.6 0.3 79.8 25.6 45.2 17.1 19.2 9.5 0.1 0.0 0.4 0.1 0.5 0.1 0.6 0.1
Root 7.4 1.4 653.8 184.9 726.2 132.6 5.2 1.5 0.9 0.1 0.4 0.1 4.4 0.9 0.6 0.1
Flower 0.2 0.2 5.9 4.3 0.0 0.0 3.0 0.7 2.6 0.4 7.2 0.5 0.7 0.1 0.6 0.1
N. tomentosiformis Lower leaf 0.0 0.0 0.0 0.0 331.2 135.1 7.0 2.1 0.7 0.7 0.7 0.2 1.2 0.3 1.2 0.3
Upper leaf 0.0 0.0 0.0 0.0 129.2 66.5 14.7 3.6 1.5 0.9 1.9 0.5 1.8 0.5 1.6 0.3
Root 0.8 0.3 1719.7 763.5 849.9 224.8 9.2 2.0 7.0 1.0 0.6 0.2 9.5 1.9 1.1 0.3
Flower 0.0 0.0 0.0 0.0 4.8 0.0 1.1 0.6 9.8 3.5 9.3 2.7 1.1 0.1 2.3 0.1