Fig. 3
Differential expression of the three receptor genes, a G protein-coupled receptor 52 (GPCR 52), an Alpha-1A adrenergic receptor-like (ADRA1A) and a metabotropic glutamate receptor 3 (mGluR3), within set 1 (a) Log2FC gene overexpression in male COTS sensory tentacle (ST). b Gene expression (TPM) in multiple COTS tissues: female sensory tentacle (STF), female tube foot (TFF), male tube foot (TFM) male radial nerve (RNM), female radial nerve (RNF), male spine (SPM), male mouth (MOM), testis (TE), early gastrula (EG), mid gastrula (MG), oocyte (OC). c Agarose gel electrophoresis of RT-PCR amplicons for 3 differentially expressed genes in multiple tissues from two pooled male and two pooled female COTS. Tissues: tube foot (TF), sensory tentacle (ST), radial nerve (RN), cardiac stomach (CS), body wall (BW), negative control (Neg). bp, base pairs