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Table 1 Sequencing run summary of the in-house dataset

From: ddSeeker: a tool for processing Bio-Rad ddSEQ single cell RNA-seq data

 

Run 1

Run 2

Quality

Low

High

# Libraries

6

6

Clusters PF (%)

33.05

93.9

Q30 (%)

70.9

82.6

Total Reads

112993193

197600946

Valid Reads

70826303 (63%)

180526539 (91%)

# Cells (expected)

1800

1800

# Cells (ddSeeker)

≈3000

≈3000

  1. Valid reads are reads with valid barcodes and UMI. Expected number of cells is based on cell capture efficiency, as declared by Bio-Rad
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BMC Genomics

ISSN: 1471-2164

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