Fig. 1

The heat map analysis for the significant conserved gene functions based on PANTHER Overrepresentation Test. The hierarchal cluster diagram was constructed by using pheatmap clustering in R. The P-values of each annotation data set (a) Molecular Function, (b) Biological Process, (c) Cellular Component, and (d) PANTHER Protein Class) are calculated with the Bonferroni correction for multiple testing by each functional group, which reflect the significance of the difference for the enrichment value between the essential and nonessential genes. The red boxes represent that the functional group are overrepresentation, while the blue boxes represent the opposite case. Conversion P-value to -log10 (x), and get the heatmap of the converted p-value, the conversion value is thought to be 0 while the P-value is greater than 0.05, the conversion value is thought to be 10 while the P-value is less than 0.0000000001