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Fig. 3 | BMC Genomics

Fig. 3

From: Identification and characterisation of microRNAs and their target genes in phosphate-starved Nicotiana benthamiana by small RNA deep sequencing and 5’RACE analysis

Fig. 3

RT-qPCR measurement of candidate miRNA abundances in N. benthamiana a) shoots and b) roots under phosphate stress. N. benthamiana were grown as in Fig. 2. Shoots and roots were harvested. Average of target transcript abundance was calculated from three independent samples as measured by qPCR. miRNA abundance values were normalised to the Nbe-UBC21 transcript and fold-change was calculated using the delta-delta –Ct method. Candidate miRNAs (miR02-miR12) are ordered according to fold-elevation in shoots and roots. N. benthamiana PHOSPHATE STARVATION2 (PS2) is included as a phosphate starvation marker. Error bars indicate ±SE

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BMC Genomics

ISSN: 1471-2164

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