Fig. 6

Computational prediction and experimental validation of Nbe-miR399 target PHOSPHATE2 (PHO2). Top: Diagram of miR399 binding sites (red boxes) in the PHO2 5′ untranslated region (5’ UTR). Part of the PHO2 coding region (grey) and an intron (blue line) is shown. Bottom: Sequence complementarity and cleavage in the PHO2 binding sites. Sequences for the highest matching miR399 species is shown at the top and the corresponding binding site in PHO2 is shown at the bottom. Bracketed number indicates the psRNA Target finder score (see Additional file 1: Table S2). Blue arrows and numbers indicate the proportion of 5’ RACE reads cleaved at the indicated sites