Fig. 8

Cleavage of N. benthamiana blue copper binding protein (BCBP) transcript by miR398. a) Top: Diagram of BCBP showing position of miR398 binding site (red box) in the 5′ untranslated region (UTR, blue box). Part of the BCBP coding region (grey) and an intron (blue line) is shown. Bottom: Sequence complementarity and cleavage at the BCBP binding site. Bracketed number indicates the psRNA Target finder score (see Additional file 1: Table S3). Blue arrow and numbers indicate the proportion of 5’ RACE reads cleaved at the indicated site. b) Alignment of computationally identified miR398 binding sites in BCBP homologues. Highlighted regions in alignment indicate similarity between species at the six-nucleotide insert region found in Arabidopsis BCBP