Identification and characterisation of microRNAs and their target genes in phosphate-starved Nicotiana benthamiana by small RNA deep sequencing and 5’RACE analysis

Table 1 Summary of small RNA deep sequencing data in six libraries

	Leaf		Stem		Root		Total
	+P	-P	+P	-P	+P	-P	Total
Input reads	3,795,314	4,716,270	8,727,396	4,128,374	11,519,516	7,160,465	40,047,335 (a)
Input reads (non-redundant)	868,291	1,622,029	2,742,250	1,624,231	3,000,466	2,335,245	12,192,512
Filter for quality, length	2,603,939	3,690,013	5,213,478	3,394,598	6,219,796	4,222,059	25,343,883
Filter for non-structural	2,347,583	3,348,874	2,963,108	3,058,748	3,434,446	2,951,462	18,104,221 (b)
Filtered non-redundant reads	575,059	1,249,586	1,212,863	1,208,898	1,134,530	1,164,834	6,545,770
Non-redundant/ Total (b)/(a) (%)	24.5	37.3	40.9	39.5	33.0	39.5
Total reads/Input reads (%)	45.2%

ISSN: 1471-2164