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Table 2 Summary of stressor phenotypes from McFarland et al. [1] putatively matched to corresponding differentially expressed transcripts. Explanation of matches are provided in the Discussion text

From: Multiple environmental stressors induce complex transcriptomic responses indicative of phenotypic outcomes in Western fence lizard

Stressor Phenotype Transcriptional    Gene
Expression Gene Transcript Accession # Symbol
Effects of TNT Exposure
Effects on Blood Homeostasis:
  Decreased mean corpuscular hemoglobin concentration (MCHC) Increased ELG 9 homolog 3 XP-421233.2 E3LN3
  Anemia (Decreased RBCs) Increased hemoglobin alpha, adult chain 2 NP-037228 Hba1
Increased hemoglobin subunit alpha-D NP-001004375 HBAD
Increased erythrocyte membrane protein band 4.1-like 2 XP-002192849  
Decreased coagulation factor IX NP-989674.1 F9
Increased cathepsin D precursor NP-990508 CTSD
Increased chitotriosidase XP-001518594  
Energy Metabolism and Body Weight:
  Hormesis - Increase in low TNT; decreased at high TNT Decreased apolipoprotein A-I precursor NP-990856.1 APOA1
Decreased apolipoprotein A-V XP-417939.2 APOA5
Decreased apolipoprotein B NP-001038098.1 APOB
Increased chitotriosidase XP-001518594  
Increased pyruvate kinase NP-990800.1 FKM
Increased glucose phosphate isomerase NP-001006128.1 GPI
Increased ubiquitin carboxyl-terminal esterase L1 NP-001073681.1 UCHL1
Increased ubiquitin specific protease 4 NP-001006134.1 USP4
Increased CNDP dipeptidase 2 NP-001006385.1 CNDP2
Increased peroxisome proliferative activated receptor y XP-416082.2  
Effects of Malaria Infection
Immune Response:
  Increased WBCs, Lymphocytes & Monocytes Increased major histocompatibility complex (MHC) NP-032235.1 Mr1
  Increased spleen size Increased CD74 molecule MHC class II invariant NP-001001613.1 CD74
Increased T-cell co-stimulator NP-001093758.1 ICOS
Increased linker for activation of T-cells family member 2 NP-001007483.1 LAT2
Increased T-cell surface glycoprotein CD3 epsilon chain precursor NP-996787.1 CD3E
Increased glutathione peroxidase 1 NP-001130041.1 GPX1
Increased CD8 antigen alpha polypeptide NP-990566.1 CD8A
Increased interferon regulatory factor 1 NP-001152868.1 Irf 1
Increased interferon regulatory factor 1 isoform a XP-002188628.1  
Increased interferon-gamma-inducible p47 GTPase XP-001377372.1  
Increased pyruvate kinase NP-990800.1 PKM
Increased glucose-6-phosphate 1-dehydrogenase NP-058702.1 G6pd
Increased interferon-gamma XP-001377372.1  
Increased interleukin 2 receptor NP-990197.1 BCL2A1
Effects of Food Limitation
Systemic Cellular Energy:
  Decreased Body Weight Decreased NADH dehydrogenase: ubiquinone NP-001006518.1 NDUFS1
  Decreased inguinal fat, brain and testes weights Decreased short-chain acyl-CoA dehydrogenase NP-001006193.1 ACADS
  Decreased Albumin and Choleserol Decreased ATP synthase XP-422453.1 ATPAF1
Increased peroxisome proliferator-activated receptor binding protein XP-418125.2 MED1
Increased apolipoprotein A-I precursor NP-990856.1 APOA1
Immune Response:
  Decreased WBCs, Lymphocytes, Eosinophils & Basophils Generalized Responses diminished energy and/or general stressor mediated decrements to standing immunity   
Interactive Effects -TNT and Food Limitation
Body Weight Change and Inguinal Fat Bodies
  TNT-induced hormesis on body weight was abolished under food restriction. TNT Increase (nullified) annexin A1 NP-996789.1 ANXA1
TNT Increase (nullified) annexin A2 NP-990682.1 ANXA2
  Reduced inguinal fat in food-restricted lizards was abolished by TNT exposure. Decrease (enhanced) apolipoprotein A-I precursor NP-990856.1 APOA1
Decrease (enhanced) apolipoprotein B NP-001038098.1 APOB
Interactive Effects - Malaria Infection and Food Limitation
WBCs /Immune Response -
  Decreased WBCs & Lymphocytes under food limitation were Increased (enhanced) CD74 molecule MHC class II invariant NP-001001613.1 CD74
  increased up to ad libitum levels under malaria infection Increased (enhanced) T-cell co-stimulator NP-001093758.1 ICOS
Increased (enhanced) linker for activation of T-cells family member 2 NP-001007483.1 LAT2
Increased (enhanced) T-cell surface glycoprotein CD3 epsilon chain precursor NP-996787.1 CD3E
Increased (enhanced) CD8 antigen alpha polypeptide NP-990566.1 CD8A
Increased (enhanced) interferon regulatory factor 1 NP-001152868.1 Irf 1
Increased (enhanced) interferon regulatory factor 1 isoform a XP-002188628.1  
Increased (enhanced) interferon-gamma-inducible p47 GTPase XP-001377372.1  
Increased (enhanced) interferon-gamma XP-001377372.1  
Increased (enhanced) interleukin 2 receptor NP-990197.1 BCL2A1
Interactive Effects - TNT and Malaria Infection
WBCs /Immune Response
  Both TNT and Malaria caused increased WBCs Increased (enhanced) B-cell linker protein NP-990239.1 BLNK
Increased (enhanced) T-cells family member 2 NP-001007483.1 LAT2
Testes Size
  TNT x Malaria reduce testes size nearly 70% - No testes expression, but systemic energy effects Decreased apolipoprotein A-I precursor NP-990856.1 APOA1