Fig. 4

Example of the TNF locus. a: TNF locus. Example of an LPS-increased H3K27ac region as well as RELA binding just downstream of the TNF gene. b: TNF expression. Detroit 562 cells were pre-treated with BAY 11–7082 or DMSO before stimulation with LPS for 100 min, RNAs were extracted and RT-qPCR performed. Results show the log 2 fold change in gene expression over control in both conditions for 3 independent experiments. c: H3K27ac mark and RELA binding at the TNF locus. Detroit 562 cells were pre-treated with BAY 11–7082 or DMSO before stimulation with LPS for 80 min and ChIP-qPCR was performed for RELA (green – primers amplifying the green region in A) and H3K27ac (orange – primers amplifying the orange regions in a). Results show the percentage of input for the two ChIPs of two independent experiments