Characterization of miRNA profiles in the mammary tissue of dairy cattle in response to heat stress

Table 2 Statistics of miRNA-seq data for control and heat stressed dairy cow mammary gland

Sample	Total reads	High quality reads	3′ adapter null	Insert null	5′ adapter contaminants	Clean reads
1	23,740,518	23,684,283	352,584	149,789	10,795	22,775,357
2	18,179,219	18,138,345	266,264	78,422	7726	17,380,420
3	21,734,611	21,684,303	324,037	64,043	5398	21,042,828
4	18,781,285	18,738,780	273,308	94,522	14,550	17,894,570
5	21,752,063	21,703,699	338,405	188,847	9943	20,759,707
6	18,485,467	18,443,383	345,756	174,824	15,332	17,214,481
7	20,690,730	20,637,198	236,984	171,114	12,944	19,673,600
8	23,575,000	23,519,333	525,390	263,643	11,551	22,132,782
Sum	166,938,893	166,549,324	2,662,728	1,185,204	88,239	158,873,745
Average	20,867,362	20,818,666	332,841	148,151	11,030	19,859,218

Total reads, total sequenced reads; High quality reads, number of high quality reads with no N, no more than 4 bases whose quality score is lower than 10 and no more than 6 bases whose quality score is lower than 13; 3′ adapter null, number of reads with no 3’adaptor; Insert null, number of reads with no insertion; 5′ adapter contaminants, number of 5′ contaminants; Clean reads, number of clean reads after adaptors and contaminants are removed which are used in the following analysis