Fig. 4

KEGG pathway classification enrichment of DGEs of P. brassicae in three developmental stages. a Enriched pathways. X-axis, the significance (−Log P-value) of KEGG Pathway Enrichment was calculated by the method of hypergeometric distribution; Y-axis, the category of KEGG Pathway (a, Metabolism; b, Genetic Information Processing; c, Environmental Information Processing; d, Cellular Processes; e, Organismal Systems; f, Human Diseases). Vertical dotted lines: P = 0.01. b Eighteen DEGs from significant KEGG Pathway Classification Enrichment were randomly selected for qRT-PCR validation. The relative expression level of each gene was expressed as the fold change between three different samples (RS, GS, IN) in the RNA-Seq data (red line chart) and qRT-PCR data (black histogram). The P. brassicae actin gene was used as an internal control to normalize the expression. Data from qRT-PCR represent the means and standard deviations. Pearson’s correlation coefficient (R-value) was used to measure the consistency of the RNA-seq data and qRT-PCR. Up, middle and down panes, DEGs from Translation and DNA replication KEGG Pathway, Cell growth and death KEGG Pathway and Cancers KEGG Pathway, respectively. See Additional file 4: Table S2 for the information of the putative genes