Fig. 6

Effect of PI3K inhibitor treatment on the clubroot symptom of B. napus. a PI3K-treated and P. brassicae-inoculated plants showed less symptom and rich rootlets; non-inoculated plants are shown as control (CK), and plants inoculated P. brassicae showed heavy symptoms (mock). Photographs were taken at 28 dpi. Bar denotes 1 cm. b Clubroot symptoms of infected roots from three biological replicates were evaluated using the percentage of plants in individual disease classes and disease index (DI) at 21 dpi and 28 dpi. Asterisks indicate statistically significant differences at the level of P = 0.05. M: Mock-treated P: PI3K inhibitor-treated. c At 28 dpi, the infected roots of mock-treated plants and inhibitor-treated plants were harvested. The relative accumulated amount of pathogen DNA was quantified by qPCR. The actin gene of B. napus was set as the control to normalize the accumulation amount of the pathogen. M, mock treated; P, PI3K inhibitor-treated. d The expression levels of genes from PI3K-related pathways between mock-treated and inhibitor-treated groups. The P. brassicae actin gene was used as an internal control to normalize the expression level. The expression level of inhibitor-treated groups was set as 1.0. Data represent the means and standard deviations