Fig. 4

Long term drought stress. Esi3 response to mild and severe drought stress in (a) the T. turgidum cultivar Creso, (b) the T. aestivum cutivar Chinese Spring (CS) and (c) a Chinese Spring line with a deletion in the 5AL chromosome (CS_5AL-10). Esi3 expression was quantified with Affymetrix microarrays, Esi3–10 is not represented on CS and CS_5AL-10 graphs since values are zero. Plants were grown as described by Aprile et al., 2009 [32]; briefly, soil grown plants were treated at anthesis at different levels of stress: Control field capacity 28% water content, mild stress 18% water content or severe stress at 12.5% water content. Background of 3.3 subtracted from all values, values are in log₂ units. Letters signify Duncan’s multiple range values