Fig. 4
Validation of RNA sequencing results by RT-qPCR. a Correlations between gene expression levels measured by RT-qPCR and RNA-Seq methods. b Comparison of RNA-Seq log2FoldChange read counts with log2FoldChange RT-qPCR copy numbers. The upper panel shows the RNA-Seq read counts (log2FoldChange) for seven genes, of which five are upregulated in chronic hypoxia vs. normoxia in L. mandarinus and L. brandtii brain and two are downregulated. The lower panel shows log2FoldChange by RT-qPCR copy numbers for the same genes in chronic hypoxia vs. normoxia in L. mandarinus and L. brandtii brain samples. (LM: L. mandarinus; LB: L. brandtii)