Fig. 3

Detailed traces of putative intrinsic disorder drawn by the “Trace” module (a-c). Extensive regions of disorder are apparent from the disorder trace of human CDC7 kinase (a). The disorder trace of the highest-scoring hit in the BASILIScan search, the CDC7 kinase homologue of E. cuniculi, reveals a much more ordered appearance (b). Disorder trace alignment module allows for identification of the disordered regions in human CDC7 (blue trace) that are missing in the fungal homologue (orange trace). The resulting structured domains are highlighted in cyan, purple and green (c). A deletion construct of human CDC7 kinase composed of the regions highlighted in panel c has been successfully crystallised and its structure solved to 2.3 Å resolution (PDB: 4F99) [26] (d) Regions of the structure in panel D are coloured corresponding to the highlighting colour in panel c