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Table 2 RNA-seq statistics

From: Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action

 

dsmalE

dsCBP

Samples

MD1-MD3

MJ1-MJ3

MT1-MT3

CD1-CD3

CJ1-CJ3

CT1-CT3

Total no. of reads

90,280,120

64,052,276

86,015,875

98,406,728

100,327,204

94,045,683

No. of high-quality reads

80,437,710

57,466,708

54,928,048

86,047,960

79,067,179

79,188,207

Mapped reads to Exona

75,27,476

53,578,082

48,830,575

81,330,901

73,271,933

71,579,430

Percent reads mapped

83.59

93.29

87.89

93.36

92.97

90.37

Uniquely mapped readsb

70,309,618

49,895,642

45,547,725

75,753,713

68,994,581

67,063,490

  1. aExon regions of Tribolium castaneum genome
  2. bReads considered for downstream gene expression analysis
  3. The table is showing a summary of read statistics after Illumina Hi-seq 4000 sequencing. Total read counts for three biological replicates from each treatment varies within 64,000,000 to 101,000,000. Number of high quality reads indicates the number of reads left after read trimming and filtering (Quality control step). Percentage of high quality reads unambiguously mapped to reference Tribolium genome represented in the second last row. Uniquely mapped reads were considered for differential gene expression analysis. Further details of RNA-seq statistics per sample are given in Additional file 1: Table S2