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Fig. 4 | BMC Genomics

Fig. 4

From: Complex regulation of microRNAs in roots of competitively-grown isogenic Nicotiana attenuata plants with different capacities to interact with arbuscular mycorrhizal fungi

Fig. 4

Investigation of reprograming of abundance of novel miRNAs in EV and irCCaMK plants by AMF inoculation. a Unsupervised hierarchical clustering of normalized miRNA abundance values was performed using the same methods as for the conserved miRNAs. The heatmap shows large number of novel miRNAs are enriched in the presence of AMF in EV (as shown by the bottom red color block). Similarly, a large number of novel miRNA reads were over-expressed in the absence of AMF in irCCaMK (-AMF) plants as shown in the upper red colored block in the heatmap. b Expression range of novel miRNAs in all the four conditions. Overall novel miRNAs have lower abundance values as compared to the conserved ones. The individual RPM values of each miRNA (in all four conditions) are provided in SI Table 1. c-f Differential expression of novel miRNAs in the pairwise comparisons of four conditions computed at a p-value < 0.05 and fold-change cut-off of > 1.2 or < − 1.2. The bar-charts for each comparison plot the up- and down-regulated differentially expressed miRNAs (DE-miRs). Red color represents up- and blue denotes down-regulation

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