Fig. 4
a. Pathway analysis of negatively correlated target mRNAs (n = 130) for differentially expressed C24MC-associated miRNAs presenting a decline in their expression during the course of gestation. Target mRNAs were mainly associated with cardiovascular development and function, and included endothelial cell movement/migration, angiogenesis, differentiation of angioblasts, formation of vascular plexus, and vascularization and morphology of blood vessels. Specific C24MC-associated miRNAs and direct relationships are shown in blue. Relevant physiological functions/pathways involved and their direct relationships are depicted with varying sizes according to the associated number of target mRNAs (ie. larger size indicative of higher number of mRNAs involved). The interactive figure can be accessed here. (http://rpubs.com/pouyadini/431871​) b. Expression patterns of mRNAs (n = 29 putative targets), which presented a significant negative correlation to their miRNA expression during gestation and are predicted to be involved in angiogenesis. The heatmap was constructed using FPKM values and scaled for each mRNA. The interactive heatmap can be accessed here. (http://rpubs.com/pouyadini/339221) c. Expression patterns of miRNAs (n = 8), which were predicted to be involved in angiogenesis in CAM during different stages of pregnancy (n = 29 samples). The heatmap was constructed using -ΔCT values and scaled for each miRNA. The interactive heatmap can be accessed here. (http://rpubs.com/pouyadini/391422​) d. Expression patterns of putative mRNA targets involved in angiogenesis (n = 10) during the course of gestation in CAM (n = 29 samples). The heatmap was constructed using -ΔCT values and scaled for each mRNA. The interactive heatmap can be accessed here. (http://rpubs.com/pouyadini/391679​)