Kinetics of the chromosome 14 microRNA cluster ortholog and its potential role during placental development in the pregnant mare

Table 7 Putative mRNA:miRNA binding site and the free energy (ΔG)

Target mRNA	MIRNA ID	ΔG	3′ UTR binding site (5′ to 3′)	Position at 3′UTR	MIRNA seed sequence	Seed sequence position
ACVRL1	hsa-miR-1247-3p	−25.4	UCCUGGG	799-805	CCCGGGA	2-8
ACVRL1	hsa-miR-1247-3p	−20.6	CCUGGG	590-595	CCCGGG	2-7
ACVRL1	eca-miR-485-5p	−26.2	GCCAGCCUC	560-568	GAGGCUGGC	2-10
ACVRL1	eca-miR-485-5p	−23.1	AGCCUC	455-460	GAGGCU	2-7
AXL	eca-miR-432-5p	−25.3	UCUAAGA	878-884	UCUUCU	1-7
AXL	eca-miR-432-5p	−22.7	GCUCUAA	1073-1080	UUGGAGU	3-9
AXL	eca-miR-432-5p	−21.8	AUUCCAAGG	1245-1254	UCUUGGAGU	1-9
AXL	eca-miR-433-3p	−20.4	CAUGAU	495-502	AUCACG	1-6
CXCL10 ^a	eca-mR-411-3p	–	–	–	–	–
DVL1	hsa-miR-1247-3p	−32.2	CUCGGGG	441-447	CCCGGG	1-7
EDNRA	eca-miR-485-3p	−12.6	UGUGAC	100-105	GUCAUA	1-6
EPN2	hsa-miR-1247-3p	−27.4	CCGGGG	331-336	CCCGGGA	1-6
EPN2	hsa-miR-1247-3p	−26.9	CCUGGGG	2087-2093	CCCCGGGA	1-7
EPN2	hsa-miR-1247-3p	−25.4	UCUGGGG	366-372	CCCCGGGA	1-7
G6PD	eca-miR-485-5p	−27.9	CCAGCCUC	409-416	GAGGCUGG	2-9
G6PD	eca-miR-485-5p	−23.2	GGCCUC	99-105	GAGGCU	2-7
G6PD	eca-miR-485-5p	−20.9	GUCUGUCUCU	35-44	AGAGGCUGGC	1-10
G6PD	eca-miR-485-5p	−16.4	CCAGUUUC	514-521	GAGGCUGG	2-9
JAM2	eca-miR-382-5p	−20.4	GAGCAAUU	1131-1155	AGUUGUUC	3-10
NRP1	hsa-miR-1247-3p	−29.5	CCCGGG	873-879	CCCGGG	2-7
NRP1	hsa-miR-1247-3p	−21.5	CCCGGG	821-826	CCCGGG	2-7
TYRO3	eca-miR-485-5p	−18	AGUCUCU	899-905	AGAGGCU	1-7

^aBinding site and free energy estimation not provided due to the lack of annotation of the 3′ UTR in the current equine genome assembly

ISSN: 1471-2164