Fig. 7

Targeting of BMP7 by hdh-miR-1984. Luciferase activity assay of the wild-type (WT) or mutant (MUT) 3′ UTR of BMP7 using a dual luciferase reporter system in 293 T cell lines following co-transfection with hdh-miR-1984 mimic, mimic Ncontrol, inhibitor, inhibitor Ncontrol, and blank control. In addition, luciferase activities significantly decrease in the BMP7-WT plasmid-transfected 293 T cells following co-transfection with hdh-miR-1984 mimic. Effects are blocked in the mutant plasmids transfected 293 T cells and in the BMP7-WT plasmids transfected 293 T cells following co-transfection with hdh-miR-1984 inhibitor. Data are derived from triplicate transfectants of three independent experiments (** P < 0.01)