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Fig. 1 | BMC Genomics

Fig. 1

From: SNP-ChIP: a versatile and tag-free method to quantify changes in protein binding across the genome

Fig. 1

SNP-ChIP adds the ability to measure semi-quantitative amounts of target protein to traditional ChIP-seq. a Main steps of SNP-ChIP exemplified for two hypothetical conditions. b Target protein Red1 levels produced by SNP-ChIP (equivalent to the wild type-normalized spike-in normalization factor), compared to previously published levels measured by western blot (mean +/− S.E.M.) [19]. Points represent individual SNP-ChIP-derived replicate values and bars represent average value. c Fragment pileup produced using MACS2 with SPMR (fragment pileup per million reads) sequencing depth normalization of an example chromosome and chromosomal region before (top panel) and after (bottom panel) spike-in normalization. d Target protein Red1 levels for Red1 dosage strain series compared to previously published levels measured by western blot (mean +/− S.E.M.) [19], as in (b)

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