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Table 2 GOPHER output files: Seven files can be exported from GOPHER via the Export menu. These files can be used for downstream analyses, documentation, visualization, and ordering of probes. The file prefix <PREFIX> corresponds to the name of the project. The prefix of the digest file additionally contains the tag for the genome build

From: GOPHER: Generator Of Probes for capture Hi-C Experiments at high Resolution

Filename

Description

<PREFIX>_DigestedGenome.txt

Content of lines: Coordinates, length, GC and repeat content, indication of selection, number of probes for each digest.

 

Purpose: Can be used for downstream analyses.

<PREFIX>_viewPoints.tsv

Content of lines: Gene symbol, coordinates, UCSC custom URL, number of selected digests, score, length, indication whether digest with TSS is selected.

 

Purpose: Documentation and sharing of results.

<PREFIX>_uniqueTargetDigests.bed

Content of lines: Coordinates and names of targeted digests. Duplicates arising from overlapping viewpoints are removed.

 

Purpose: Probe design with external tools.

<PREFIX>_uniqueTargetDigestMargins.txt

Content of lines: Coordinates and names of margins of targeted digests.

 

Purpose: Probe design with external tools.

<PREFIX>_allTracks.bed

Content of lines: Contains multiple sections for viewpoints, digests, margins, and probes.

 

Purpose: Can be uploaded as multiple custom tracks to UCSC.

<PREFIX>_ProbeFile.bed

Content of lines: Coordinates and names of probes.

 

Purpose: Ordering of probes.

<PREFIX>_agilentProbeFile.txt.zip

Content of lines: Coordinates, names, and sequences of probes.

 

Purpose: Ordering of probes.