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Table 1 The number of differentially expressed transcripts detected by the Trad-KAPA and 3’-LEXO, before and after subsampling from 10 million reads

From: A comparison between whole transcript and 3’ RNA sequencing methods using Kapa and Lexogen library preparation methods

Sequencing Depth Trad-KAPA Intersection (with 10 m) 3’-LEXO Intersection (with 10 m) Intersection (Trad-KAPA and 3’-LEXO)
1 million 343 339 (98.8%) 257 249 (96.9%) 177
2.5 million 758 742 (97.9%) 474 460 (97.0%) 329
5 million 1234 1194 (96.8%) 777 740 (95.2%) 562
10 million 1982 1982 1157 1157 882
  1. The first column denotes the sequencing depth (i.e. the total number of mapped reads from the library examined). The second column denotes the number of differentially expressed transcripts detected by Trad-KAPA. The third column denotes the number of differentially expressed transcripts detected after subsampling that overlap with those from the 10 million sequencing depth. The fourth and fifth columns denote the results for the 3’-LEXO method. The sixth column denotes the number of differentially expressed transcripts detected by both the Trad-KAPA and the 3’-LEXO methods at listed sequencing depth