Gene name | Primer set used | RT-qPCR fold change | Trad-KAPA fold change | 3’-LEXO fold change | RT-qPCR result match which RNA-Seq method | Group |
---|---|---|---|---|---|---|
Adnp | mAdnp-ex2–3 | 0.83 | 1.15 | 5.02 | Trad-KAPA | Trad-KAPA only |
Cd7a | mCd7a-ex3–4 | 0.69 | 0.79 | 5.11 | Trad-KAPA | Trad-KAPA only |
Fv1 | mFv1-F169 | 0.55 | 0.54 | 10.48 | Trad-KAPA | Trad-KAPA only |
Mid1 | mMid1ex4–5 | 0.77 | 0.53 | 5.12 | Trad-KAPA | Trad-KAPA only |
Mid1 | mMid1ex8–9 | 0.83 | 0.53 | 5.12 | Trad-KAPA | Trad-KAPA only |
Mmp28 | mMmp28ex2–3 | 3.24 | 4.52 | 8.55 | Trad-KAPA | Trad-KAPA only |
Unkl | mUnkl-ex5–6 | 0.75 | 1.11 | 5.12 | Trad-KAPA | Trad-KAPA only |
Unkl | mUnkl-ex2–3 | 0.90 | 1.11 | 5.12 | Trad-KAPA | Trad-KAPA only |
Zfp647 | mZfp647-204ex4–5 | 0.55 | 0.42 | 8.46 | Trad-KAPA | Trad-KAPA only |
Zfp647 | mZfp647-201ex3–4 | 0.59 | 0.42 | 8.46 | Trad-KAPA | Trad-KAPA only |
Bcl2a1b | mBcl2a1bEx1–2 | 2.91 | 1.44 | 5.76 | In between | 3’-LEXO only |
Hist4h4 | mHist4h4 | 1.83 | 0.26 | 0.27 | Neither | 3’-LEXO only |
Mir5136 | mMir5136 | 1.47 | 5.07 | 0.88 | 3’-LEXO | 3’-LEXO only |
Mt-Tq | mMt-Tq | 1.09 | 0.95 | 0.30 | Trad-KAPA | 3’-LEXO only |
Rps27rt | mRps27rt | 1.27 | 0.27 | 1.40 | 3’-LEXO | 3’-LEXO only |
S100a4 | mS100a4ex1–2 | 1.93 | 1.42 | 2.31 | 3’-LEXO | 3’-LEXO only |
S100a4 | mS100a4ex2–3 | 2.06 | 1.42 | 2.31 | 3’-LEXO | 3’-LEXO only |
Schip1 | mSchip1ex7–8 | 0.85 | 0.51 | 0.90 | 3’-LEXO | 3’-LEXO only |
Snord118 | mSnord118 | 0.46 | 0.26 | 0.60 | 3’-LEXO | 3’-LEXO only |
Snord13 | mSnord13 | 0.92 | 0.98 | 0.48 | Trad-KAPA | 3’-LEXO only |
Spink1 | mSpink1ex3–4 | 9.49 | 2.66 | 8.28 | 3’-LEXO | 3’-LEXO only |
Tceal5 | mTceal5ex3–4 | 5.82 | 10.48 | 30.09 | Trad-KAPA | 3’-LEXO only |
Tceal5 | mTceal5ex1–2 | 6.07 | 10.48 | 30.09 | Trad-KAPA | 3’-LEXO only |
Atoh8 | mAtoh8 | 3.97 | 3.10 | 3.19 | Both | Iron metabolism |
Bdh2 | mBdh2 | 0.28 | 0.35 | 0.39 | Both | Iron metabolism |
Bmp6 | mBmp6 | 4.83 | 6.01 | 6.20 | Both | Iron metabolism |
Cp | mCp | 1.77 | 1.88 | 1.94 | Both | Iron metabolism |
Ftl1 | mFtl1 | 1.75 | 0.98 | 3.26 | In between | Iron metabolism |
Hamp1 | mHamp1 | 5.75 | 5.19 | 5.73 | Both | Iron metabolism |
Hamp2 | mHamp2 | 0.26 | 0.28 | 0.33 | Both | Iron metabolism |
Hfe2 | mHfe2 | 0.61 | 0.66 | 0.67 | Both | Iron metabolism |
Id1 | mId1F205&200 | 4.05 | 3.43 | 3.19 | Both | Iron metabolism |
Lcn2 | mLcn2 | 2.91 | 2.92 | 2.25 | Both | Iron metabolism |
Slc11a2 | mSlc11a2 | 0.66 | 0.80 | 0.73 | Both | Iron metabolism |
Smad7 | mSmad7 | 3.33 | 3.85 | 2.91 | In between | Iron metabolism |
Tfrc | mTfrc | 1.16 | 1.26 | 1.32 | Both | Iron metabolism |