Fig. 6From: Chromosome rearrangements shape the diversification of secondary metabolism in the cyclosporin producing fungus Tolypocladium inflatumTranslocation of a T1-PKS and several accessory genes between chromosomes. a Three PKS clusters (12, 13, and 14; red bars below chromosome) were moved as part of the large 800 Kb translocation of the end of chromosome 2 (orange) to the end of chromosome 6 (dark-blue) in 8044 (Fig. 3; I). Within this translocated region in 8044, however, an additional cluster (cluster E) was identified that was not found in any other strains and contained a T1-PKS gene that shared homology with a T1-PKS in cluster 27 on chromosome 4 and appears to have been translocated from chromosome 4 to chromosome 6 (Fig. 3; II). Between panels B and C is a cartoon of the end of chromosome 6 in 8044, showing segments translocated from chromosome 2 (orange) and chromosome 4 (green). Above this cartoon is a zoomed in genomic alignment of the region surrounding cluster E (red bar containing genes as yellow (T1-PKS) or blue (accessory gene) with b syntenic regions from chromosome 2 in all other strains (above, orange) showing that the T1-PKS and accessory genes are not found on chromosome 2 in other strains. Below the cartoon is a genomic alignment of the translocated region surrounding cluster E on chromosome 6 in 8044 with (c) the region surrounding cluster 27 on chromosome 4 (below, green) in all other strains. Only the green segment containing the T1-PKS and accessory genes aligns with the PKS and homologous accessory genes in cluster 27 on chromosome 4, but in an inverted orientation. Each genomic alignment shows the chromosome as a line, genes as blue boxes in a track below the line, SMBGCs as a red box in the gene track, and TEs as green blocks and arrows. Colored shaded bars directly above (forward) or below (inverted) the line indicate syntenic blocks. Red shading connecting the gene tracks indicates genes shared within cluster E, while grey shading between gene tracks indicates shared genes flanking the clusterBack to article page