Fig. 6

qRT-PCR validation of the expression level of predicted target genes and corresponding miRNAs under Sporisorium scitamineum stress at different process times. a Expression analysis of miR529-3p, miR168a-5p, miR160a, and their predicted target genes UCH-L5, AGO 1B, and ARF8. b Expression analysis of miR5293, nov-mir-132, miR162a, and their predicted target genes AIP, CCR, and SAMDC. c Expression analysis of nov-mir-143, miR5368, and their predicted target genes EIL3 and HIR1. d Expression analysis of nov-mir-63, nov-mir-97, nov-mir-10, miR858b, nov-mir-18, miR396e-5p, nov-mir-66, and their predicted target genes GK, PP2C, MLO, MYB2, and GRF8. All data points were means ± standard error (n = 3). Different lowercase letters indicated a significant difference, as determined by the Duncan’s new multiple range test (p < 0.05). YC05–179, smut-resistant genotype; ROC22, smut-susceptible genotype. UCH-L5, ubiquitin carboxyl-terminal hydrolase isozyme L5-like; AGO 1B, protein argonaute 1B; ARF8, auxin response factor 8; AIP, auxin-induced protein; CCR, cinnamoyl-CoA reductase; SAMDC, S-adenosylmethionine decarboxylase; EIL3, ethylene-insensitive 3-like 3 protein; HIR1, hypersensitive-induced response protein 1; GK, glycerol kinase; PP2C, protein phosphatase 2C; MLO, MLO-like protein; MYB2, Myb-related protein Hv33; GRF8, growth-regulating factor 8