Skip to main content

Advertisement

Fig. 2 | BMC Genomics

Fig. 2

From: Chronic circadian misalignment results in reduced longevity and large-scale changes in gene expression in Drosophila

Fig. 2

CCM causes aberrant locomotor activity behavior. a-d Average activity profiles for the indicated groups and time points depict the number of DAM beam breaks/min in 30 min bins for an entire light-dark cycle (which consisted of 24 h for controls and 28 h for CCM flies) averaged across one week (seven 24-h days for control or six 28-h days for CCM flies). White and black bars correspond to light and dark periods, respectively. Error bars represent standard error of the mean (SEM). ZT refers to zeitgeber time, with ZT0 being the time of lights-on. e-f Weekly activity levels in mean beam breaks/min ± SEM are shown for female (e) and male (f) flies for weeks 1, 2 and 3 and across the full life of the animals. For females, 2-Way ANOVA indicated a significant effect of time (F(3, 869) = 15.1, p = 1.47E-9) but not treatment (F(1, 869) = 0.149, p = 0.700) and Sidak’s posthoc test revealed no differences between control and CCM-exposed flies for any time point (p ≥ 0.237 for all). For males, 2-Way ANOVA indicated a significant effect of time (F(3, 831) = 15.2, p = 1.25E-9) and treatment F(1, 831) = 17.9, p = 2.61E-5) and Sidak’s posthoc test revealed a significant difference between control and CCM-exposed flies for week 2 (p = 6.97E-5) and full life (p = 8.30E-3) but not week 1 (p = 0.063) or week 3 (p = 0.999)

Back to article page