Fig. 3
CCM reduces LD rest:activity rhythm strength but does not affect free-running rhythms. a-b Mean rest:activity rhythm power ± SEM is shown for female (a) and male (b) flies for each week of the experiment during exposure to control (blue) or CCM (red) conditions. For female flies, 2-Way ANOVA indicated a significant main effect of time (F(3, 651) = 66.7, p < 1.0E-15) and treatment (F(1, 651) = 28.8, p = 1.09E-7) and Sidak’s posthoc test revealed significant differences between control and CCM females for weeks 1 (p = 3.72E-8), 2 (p = 4.47E-7) and 3 (p = 6.73E-3), but not for week 4 (p = 0.893). Similar results were observed in male flies, for which 2-Way ANOVA indicated a significant main effect of time (F(3, 611) = 16.7, p = 1.90E-10) and treatment (F(1, 611) = 25.6, p = 5.45E-7), and Sidak’s posthoc test revealed significant differences between control and CCM males for weeks 1 (p = 4.20E-14), 2 (p = 3.20E-9) and 3 (p = 3.39E-2), but not for week 4 (p = 0.813). For all graphs, ****p < 0.0001; **p < 0.001; *p < 0.01, Sidak’s posthoc test. c-d Rest:activity rhythm period is shown for female (c) and male (d) flies for each week of the experiment during exposure to control (blue) or CCM (red) conditions. Each dot represents the period of an individual fly and lines indicate mean ± 95% confidence intervals. (e-f) Mean free-running rest:activity rhythm power ± SEM is shown for female (e) and male (f) flies previously exposed to 1, 2 or 3 weeks of control (blue) or CCM (red) conditions. For females, 2-Way ANOVA indicated a significant effect of time (F(2, 138) = 59.1, p < 1.0E-15) but not treatment (F(1, 138) = 1.91, p = 0.169). Similarly, for males, 2-Way ANOVA indicated a significant effect of time (F(2, 119) = 22.7, p = 4.54E-9) but not treatment (F(1, 119) = 0.255, p = 0.614). g-h Free-running rest:activity rhythm period is shown female (g) and male (h) flies previously exposed to 1, 2 or 3 weeks of control (blue) or CCM (red) conditions. Each dot represents the period of an individual fly and lines indicate mean ± 95% confidence intervals. For E and G, n = 28 for 1-week control females, n = 30 for 1-week CCM-exposed females, n = 26 for 2-week control females, n = 27 for 2-week CCM-exposed females, n = 17 for 3-week control females, and n = 16 for 3-week CCM-exposed females. For F and H, n = 30 for 1-week control males, n = 30 for 1-week CCM-exposed males, n = 28 for 2-week control males, n = 26 for 2-week CCM-exposed males, n = 7 for 3-week control males, and n = 4 for 3-week CCM-exposed males. i Mean normalized activity is plotted for the last three days in LD followed by the first three days in DD for the genotypes listed. Flies were transferred to DD following 2 weeks exposure to CCM or control conditions. Data are single plotted and each line is 24 h. Dotted red lines track activity offset. n = 16 per group. Note the immediate transition to ~ 24-h free-running periods for all groups after transfer to DD