Fig. 4

CCM causes minor reductions in sleep duration. a-d Mean sleep/30 min ± SEM is shown for female (a and c) and male (b and d) flies over the course of a single light-dark cycle averaged over one week. For control flies (a and b), an average 24-h day is shown, blue bars depict sleep during week 1, green bars depict sleep during week 2 and magenta bars depict sleep during week 3. For CCM-exposed flies (c and d), an average 28-h day is shown, red bars depict sleep during week 1, orange bars depict sleep during week 2 and brown bars depict sleep during week 3. e-f Mean min sleep/hr. ± SEM is shown for female (e) and male (f) flies for weeks 1, 2 and 3 and across the full life of the animals during exposure to control (blue) or CCM (red) conditions. For females, 2-Way ANOVA indicated a significant effect of treatment (F_{(1, 866)} = 5.17, p = 2.32E-2) and time (F_{(3, 866)} = 4.38, p = 4.52E-3) and Sidak’s posthoc test revealed a significant difference between control and CCM-exposed flies for week 1 (p = 2.81E-2), but not for week 2 (p = 0.999), week 3 (p = 0.919) or full life (p = 0.584). For males, 2-Way ANOVA indicated a significant effect of treatment (F_{(1, 828)} = 63.5, p < 1.0E-15) and time (F_{(3, 828)} = 31.4, p = 5.0E-15) and Sidak’s posthoc test revealed a significant difference between control and CCM-exposed flies for week 1 (p = 2.3E-5), week 2 (p = 3.39E-13), and full life (p = 1.96E-6) but not week 3 (p = 0.973)