Fig. 1

Identified circRNAs by RNA-Seq. a Two independent algorithms were used to predict circRNAs: segemehl in conjunction with testrealign (green) reported 2725 BSJs while BWA with CIRI2 reported 1922 BSJs. The overlap was considered as low stringency set (yellow). The high stringency set additionally requires an enrichment through RNase R treatment and compelling read coverage from at least two independent sequencing libraries. See Materials and Methods for details. These 254 candidates are most likely genuine circRNAs and are therefore used for further analysis. b Strong evidence is found in three independent sequencing libraries for 139 candidates. The remaining 115 are well supported by two samples. Note that all candidates were also identified as enriched in E₊ vs E₋. c 122 host genes are orthologous to host genes of circRNAs identified in either Drosophila or Bombyx in previous studies