Fig. 2

Location of circRNAs in the original transcripts. BSJs are clustered into the following categories: part of the 5’-UTR, from the coding sequence exclusively, part of the 3’-UTR, spanning from 5’ to 3’-UTR or other (in the middle of exons, introns, part of non-coding genes or in between genes). Most of the circRNAs in A. mellifera originate from exons in the coding portion of a transcript, which also represents the largest amount of exons transcriptome-wide. Compared to exons of a random control, circRNAs originate slightly more often from 5’-UTR exons as also described for fruit fly. However, we did not find as many BSJ including exons of the 3’-UTR as reported there. All high confidence circRNAs were mapped to annotated splice sites and we could not detect BSJ with sufficient read numbers outside of annotated regions