Fig. 4
The quantitative RT-PCR analysis of gene expression before and after cold treatment
The y-axis indicates fold change in expression among the samples. Expression levels were normalized using the threshold cycle values obtained for the Ubiquitin and Actin genes. Error bars indicate the standard deviation of the mean (SD) (n = 3). Three replicates were analyzed, with similar results. One way ANOVA with Bonferroni multiple comparison test significant at P < 0.05 between the two samples
