Fig. 7From: Peptimapper: proteogenomics workflow for the expert annotation of eukaryotic genomesa Artemis view of cluster “A” for validation. Ectocarpus sp. genes close to these clusters are represented by linear exons colored in yellow. Another representation of the same exons is shown in light blue along the six reading frames (+ 1, + 2, + 3 above and − 1, − 2, − 3 below). The cluster is indicated by black bold rectangles around the small rectangles of the PSTs. b Data for cluster “A”. c Experimental validation conditions. d RT-PCR validation: agarose gel electrophoresis of RT-PCR products (lane 1), RNA-PCR negative controls (lane 2), and DNA size marker (lane 3) for PST cluster “A”Back to article page