Table 1 Comparison of tag quality between Peaks and PepNovo+, using Proteome Discoverer ™ peptide identifications as the expected sequences
From: Peptimapper: proteogenomics workflow for the expert annotation of eukaryotic genomes
Peaks | Proteome discoverer (MASCOT identifications) | Pepnovo+ | |||||||
|---|---|---|---|---|---|---|---|---|---|
ID | Tag sequence | Significant score >60% | Rank | Protein AC | Reference Sequence | e-value | ID | Tag sequence | Significant score >1,5 |
271 | WVQAAGAGASR | 23 | 0 | Esi0085_0010 | SVVQAAGAGDAK | 5,65568E-06 | Spectrum271_scans | QAAGAGA | 6,346 |
271 | WVQAAGAGWK | 21.5 | 1 | ||||||
271 | WVQAAGAGTGR | 18.5 | 2 | ||||||
552 | (CamC)VGVSEETTTRHR | 31 | 0 | Esi0091_0058 | cVGVSEETTTGVHR | 2,36909E-07 | Spectrum552_scans | GVSEDD | 4,237 |
552 | QMGVSEETTTRHR | 29 | 1 | ||||||
552 | V(CamC)GVSEETTTRHR | 17 | 2 | ||||||
577 | QFAGDDAPR | 43 | 0 | Esi0203_0038 | AGFAGDDAPR | 1,69937E-06 | Spectrum578_scans | GDDAPR | 7,539 |
577 | K(MetOxM)AGDDAPR | 41 | 1 | ||||||
577 | AGFAGDDAPR | 11.5 | 2 | ||||||
642 | KAENPMSKR | 100 | 0 | Esi0349_0012 | KAEDIDTIR | 1,01272E-05 | Spectrum643_scans | DLDTLR | 4,727 |
642 | KAQDPMSKR | 0.1 | 1 | ||||||
642 | QAKDPMSKR | 0.02 | 2 | ||||||
1118 | DGLVYGK(MetOxM)NEPPGAR | 38 | 0 | Esi0327_0021 | ATLVYGQmNEPPGAR | 2,42457E-06 | Spectrum1119_scans | EPPGAR | 1,396 |
1118 | DGLVYGQFNEPPGAR | 37 | 1 | ||||||
1118 | DGLVYGQ(MetOxM)NEPPGVK | 8 | 2 | ||||||
2462 | DESAAVFAWK | 87,5 | 0 | Esi0091_0058 | DESAAVFAWK | 1,64304E-05 | Spectrum2463_scans | SAAVFA | 4,78 |
2462 | DESAAVFAGTR | 8 | 1 | ||||||
2462 | LMSAAV(MetOxM)AGEK | 1.5 | 2 | ||||||
2479 | MDDLTNNALARK | 58 | 0 | Esi0888_0002 | mVDLTPMAIAAGR | 4,70366E-07 | Spectrum2480_scans | ALAAGR | 1,943 |
2479 | (MetOxM)VDLTNNALAAGR | 39.5 | 1 | ||||||
2479 | MDDLTNGGALARK | 1 | 2 | ||||||
2558 | ED(MetOxM)ETE(CamC)AVNYDNLYQVMK | 48 | 0 | Esi0349_0012 | TccEAEcAVNYDNLYQAMR | 1,01272E-05 | Spectrum2559_scans | VNYDNL | 2,289 |
2558 | DE(MetOxM)ETE(CamC)AVNYDNLYQVMK | 8.5 | 1 | ||||||
2558 | ED(MetOxM)ETE(CamC)AVNYDNLYQMVK | 8 | 2 | ||||||
2610 | TFQAGEVASALLGR | 32 | 0 | Esi0327_0021 | FTQAGAEVSALLGR | 5,99619E-08 | Spectrum2610_scans | SALLGR | 4,356 |
2610 | FTQAGEVASALLGR | 25 | 1 | ||||||
2610 | TFKAGAGNGSALLGR | 10 | 2 | ||||||
3191 | VALTGLTLAEYFR | 53 | 0 | Esi0327_0021 | VALTGLTIAEYFR | Spectrum3196_scans | LAEYFR | 4,471 | |
3191 | GLLTGLTLAEYFR | 25.5 | 1 | ||||||
3191 | AVLTGLTLAEYFR | 11 | 2 | ||||||